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A DNA sequence encoding the signal peptide and extracellular domain of human TNF Receptor 1 (TNFRI) (aa 1-209) was fused to the Fc region of human IgG1 (aa 93-330). The chimeric protein was expressed in modified human 293 cells.

Tumor necrosis factor alpha (TNF-a ; TNF alpha) and the structurally related lymphotoxin alpha are pro-inflammatory cytokines produced by a variety of immune and non-immune cells in response to multiple stimuli. These stimuli can include bacteria and bacterial products such as LPS, viral infections, T-cell receptor activation and cross-linking of surface immunoglobulin (Ig) on B cells. Expression of TNF-alpha has been detected in macrophages/monocytes, B-cells, T-cells, basophils, eosinophils, glial cells and mast cells. The effects of TNF-a are mediated through the TNF receptors, TNF RI (TNF R1) and TNF RII (TNF R2). These receptors are type I transmembrane glycoproteins belonging to the TNF receptor superfamily, which is defined by the presence of repeating units of cysteine clusters. TNFRI is present on virtually all cell types except for red blood cells. TNFRI is a 55 kDa glycoprotein and contains 5 N-linked glycosylation sites. For a review on TNFRI refer to Chen G & Goeddel DV (2002) Science. 296(5573): 1634-5.