Go Back FITC-labeled anti-FceRIa(human IgE receptor) antibody (CRA2)

Product Detail

FceR1a is a subunit of the high affinity receptor of IgE to which it directly binds. FceR1 is a tetrameric complex consisting of one a, one b and two additional subunits. The latter two are required for signal transduction activity. The FceR1 complex plays an important role in triggering allergic responses. The CRA2 (AER24) monoclonal antibody reacts with the FceR1a subunit on a region that overlaps the region of the IgE binding site, thus it competes with IgE for the receptor binding. Since the CRA1 (AER37) monoclonal antibody reacts with the site different from the IgE binding site on FceR1a, it does not compete with IgE for the receptor binding. Combining the two antibodies, one can quantitatively measure the amounts of the IgE-bound FceR1a. The IgG fraction is purified from serum free culture medium of mouse hybridoma (CRA2) by propriety chromatography under mild conditions. This product is a biotinylated IgG produced from the IgG fraction.

Application 1) Western blotting (1ug/ml*) 2) FACS 3) Immunohistochemistry 4) Titration of IgE-bound fraction of the FceR1a using CRA1 and CRA2 antibodies