Go Back anti-FceRIa(human IgE receptor) antibody, monoclonal(CRA2)

Product Detail

FceR1a is subunit of the high affinity receptor of IgE to which it directly binds. FceR1 is a tetrameric complex consisting of one a, one b, and two additional subunits. The latter two are required for signal transduction activity. The FceR1 complex plays an important role in triggering allergic responses. The CRA1 (AER37) monoclonal antibody reacts with the FceR1a subunit on a region that does not overlap the region of the IgE binding site, thus it does not compete with IgE for the receptor binding. Since the CRA2 (AER24) monoclonal antibody (cat.#72-005) reacts with the IgE binding site on FceR1a, it competes with IgE for the receptor binding. Combining the two antibodies, one can quantitatively measure the amounts of the IgE-bound FceR1a. This product is the IgG fraction purified from serum-free culture medium of mouse hybridoma (CRA2) by propriety chromatography under mild conditions.

Applications 1) Western blotting (1ug/ml*) 2) FACS 3) Immunohistochemistry 4) Titration of IgE-bound in combination with CRA2 antibody