Go Back anti-Rad51p(human) antibody. IgY affinity purified
Product Detail
| Cat # | 70-010 |
| Size | 50 ug |
| Price |
$900.00 |
| Downloads | |
Human Rad51 protein is a functional and structural homolog of E. coli RecA protein, which plays a major role in genetic recombination and recombination repair by mediating strand exchange reaction between homologous DNA strands. Rad51 functionally and physically interacts with its paralogs, Dmc1, Rad51B, Rad51C, Rad51D, Xrcc2 and Xrcc3, and also with Rad52 in recombination processes. It also interacts with oncogenes and tumor suppressors such as BRACA1, BRACA2, and P53 for the maintenance of genome stability. The product was purified from the eggs of immunized chicken with full-size recombinant Rap51 protein expressed in E. coli and affinity purified. Affinity purification increased antibody titer 30-fold.
Using this anti-serum, Rad51 protein (37 kD) in the crude extract was detected by Western blotting at 40 kD position and Rad51 foci formation induced by stalled replication and DNA-damage was detected by indirect immunofluorescence. This antibody was successfully used for immunoprecipitatio , western blot and foci detection.