Anti-SARS antibody (clone 3A2)

Applications: Virology/Pathology
Categories: Antibodies

A novel type of coronavirus has been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome). Spike glycoprotein is essential for the infection and directly binds to the virus receptor, ACE2 (Angiotensin-Converting Enzyme 2). Hybridoma 3A2 has been isolated by injecting mouse with SARS virus and as the clone which produces antibody that specifically reacts with the virus-infected cell, in the laboratory of Prof. K. Ikuta of Osaka University. Monoclonal antibody 3A2 recognizes the spike protein consisting of 1181 amino acids, which migrates at 200 kDa position on SDS-PAGE due to its glyco-chains. Applications: 1) Western blot 0.1~0.3 ?g/ml, 2) Immunofluorescence staining (IHC), 3) ELISA

Keywords: SARS, antibody, coronavirus