anti-E.coli RuvA antibody, rabbit serum

Applications: DNA Repair/Recombination
Categories: Antibodies

E. coli RuvA protein binds specifically to the Holliday structure which is the intermediate of recombination at the late stage of homologous recombination and recombination repair, and forms a complex with RuvB motor protein, allowing the migration of Holliday junction using ATP hydrolysis energy and expands the heteroduplex region. In solution, it forms a tetramer and binds to the cross-like DNA of the Holliday junction from below and above, holding it in between. Using this antiserum in Western blot, the band of 22kD corresponding to RuvA was obtained from the extract of E. coli cells. Applications 1) ELISA 2) Western blot x 3,000 dilution

Keywords: RuvA, antibody