Fluorescent Human Dermal Fibroblasts (TTFLUOR-HDF)
TTFFLUOR Humar Dermal Fibroblasts (HDFs) are Green fluorescent primary HDFs, which has been developed transfecting the tGFP expression vector into the cell genome. These cells are expressing the green fluorescent protein gene sequences as free cytoplasmatic protein. TTFLUOR HDFs are intended to be used in cell-based assay applications providing consistent levels of tGFP expression during 10-15 doubling populations, the estimated proliferative capacity for these primary cells.
Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. Fibroblasts secrete a nonrigid extracellular matrix that is rich in type I and/or type III collagen. Dermal fibroblasts also secrete large quantities of hyaluronan in response to inflammatory stimuli. During wound healing, dermal fibroblasts switch from a migratory, repopulating phenotype to a contractile, matrix-reassembling phenotype.