Go Back IL-4

Product Detail

A DNA sequence encoding the human Interleukin 4 protein sequence (containing the signal peptide sequence, and the mature human Interleukin 4 sequence) was expressed in modified human 293 cells.

Interleukin 4 (IL-4) is a cytokine that regulates the development, proliferation and maturation of B and T-lymphocytes, as well as non-leukocyte somatic cells. IL-4 promotes antigen presentation on B cells by enhancing expression of class II MHC molecules and the co-stimulatory molecules CD80 and CD86. IL-4 can also enhance the secretion and cell surface expression of IgE and IgG1 on B-cells. The expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes is regulated by IL-4. IL-4 is produced by a number of hematopoietic cell types including T-cells (predominantly TH2 cells), CD3+ NK-T cells, mast cells, eosinophils, and basophils. Human IL-4 is synthesized as a precursor protein of 153 amino acids, which includes a hydrophobic signal sequence of 24 amino acids that is cleaved upon secretion from the cell. Biological activity of IL-4 is dependent on the formation of 3 disulfide bridges and its structure is related to that of GM-CSF, M-CSF, and growth hormone. For a review on IL-4 and its impact on normal and leukemic CLL B-cells please refer to Kay NE & Pittner BT (2003) Leuk Lymphoma. 44(6): 897-903.