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A DNA sequence encoding the signal peptide and extracellular domain of human FGF R1 alpha (IIIc) (aa 1-363) was fused to the Fc region of human IgG1 (aa 93-330). The chimeric protein was expressed in modified human 293 cells.

Fibroblast growth factors (FGFs) belong to a large family (23 human members) of mitogenic factors that range in molecular mass from 17-34kDa. FGFs exhibit high affinity for heparin and heparin like glycosaminoglycans (HLGAGs), and these interactions are required for signaling activation from the FGF receptors. FGFs are mitogens for fibroblasts and many other cell types and have additional cellular functions including effects on differentiation, survival and motility. FGFs have major roles in hematopoiesis, development, wound repair, angiogenesis and tumor growth. During embryonic development, FGFs are involved in the formation of epithelial tissues, organs and limbs. In adult organisms FGFs play a role in tissue repair and response to injury. The biological actions of FGFs are mediated through binding to the cognate FGF receptors, which are members of the tyrosine kinase receptor family. There are multiple receptor splice variants that produce a number of isoforms for each of the receptors. The FGF receptors are transmembrane cell surface proteins that comprise an extracellular domain composed of three immunoglobulin-like (Ig) loops, a transmembrane segment and an intracellular tyrosine kinase domain. Human fibroblast growth factor receptor 1 (FGF R1) is synthesized as an 882 amino acid type I membrane glycoprotein. One splice variant uses the alternatively spliced exon 9 to encode the IIIc-type carboxyl terminus for the third Ig domain, resulting in the isoform FGF R1 alpha. FGF R1 alpha (IIIc) Chimera is expressed predominantly in skin, brain, and lung tissues and binds several FGFs including FGF-1, 2, 4, 5, 6, and 9. There are 9 potential N-linked glycosylation sites in FGF R1 alpha (IIIc). For a review on FGF receptor signaling please refer to Eswarakumar VP, Lax I, Schlessinger J. (2005) Cytokine Growth Factor Rev. 16(2): 139-49 and Ezzat S and Asa SL (2005) Horm Metab Res. 37(6):355-60.