Go Back Red FLIVO In Vivo Apoptosis Kit--SR-FLIVO

Product Detail

FLIVO in vivo Apoptosis Kits provide a simple yet accurate method to detect apoptosis in vivo. The non-cytotoxic FLIVO (FLuorescence in vIVO) probes are injectable fluorescent inhibitors of caspase activity. Upon intravenous injection, the FLIVO probes preferentially form covalent bonds with active caspases, causing cells undergoing apoptosis to fluoresce. The bound FLIVO probe will remain inside the cell as long as the cell membrane is intact. Any unbound FLIVO is removed with the natural circulation of the animal. The resulting fluorescent signal within samples is a direct measure of apoptosis that occurred at the time the reagent was injected.

Once unbound FLIVO reagent has been allowed to clear from the non-apoptotic tissues, the tissues are ready for analysis and no further staining is necessary. Because FLIVO is a direct stain, it eliminates any false positives that may arise from ex vivo manipulation of the tissue. This gives a true representation of the induction of apoptosis in vivo as a result of the experimental condition.

FLIVO may be used in animal models to monitor the efficacy of treatment or the effects of disease. Among its demonstrated uses, it has been used successfully in murine models of cancer, rat brain studies, and avian brain studies (see citations below). FLIVO is non-toxic, cell-permeant, and crosses the blood-brain barrier. Reagent titration experiments should be performed to determine the amount of reagent that will work best for the size of the animal and the target tissue type.

SR FLIVO has a red fluorescent label, sulforhodamine B, with an optimal excitation range of 560 - 565 nm and optimal emission range of 585 - 595 nm. A green carboxyfluorescein-labeled FLIVO probe is also available. Products are available in small kits (enough for 6 rats or mice) and large kits (enough for 24 rats or mice).