Go Back Green FLIVO In Vivo Apoptosis Kit--FAM-FLIVO

Product Detail

FLIVO in vivo Apoptosis Kits provide a simple yet accurate method to detect apoptosis in vivo. The non-cytotoxic FLIVO (FLuorescence in vIVO) probes are injectable fluorescent inhibitors of caspase activity. Upon intravenous injection, the FLIVO probes preferentially form covalent bonds with active caspases, causing cells undergoing apoptosis to fluoresce. The bound FLIVO probe will remain inside the cell as long as the cell membrane is intact. Any unbound FLIVO is removed with the natural circulation of the animal. The resulting fluorescent signal within samples is a direct measure of apoptosis that occurred at the time the reagent was injected.

FLIVO is a direct stain and eliminates false positives that may arise from ex vivo manipulation of tissue. This gives a true representation of the induction of apoptosis in vivo as a result of the experimental condition.

FLIVO may be used in animal models to monitor the efficacy of treatment or the effects of disease. Among its demonstrated uses, it has been used successfully in murine models of cancer, rat brain studies, and avian brain studies (see citations and data below).

FLIVO is non-toxic, cell-permeant, and crosses the blood-brain barrier. Reagent titration experiments should be performed to determine the amount of reagent that will work best for the size of the animal and the target tissue type.

FAM FLIVO has a green fluorescent label, carboxyfluorescein, with an optimal excitation range from 490 - 495 nm and optimal emission range from 515 - 525 nm. A red-labeled SR-FLIVO probe is also available. Products are available in small kits (enough for 6 rats or mice) and large kits (enough for 24 rats or mice).