Go Back Green FLICA Caspase 13 Assay Kit--FAM-LEED-FMK

Product Detail

Detect active caspase-13 with the FAM FLICA Caspase 13 Assay Kit. This in vitro assay employs the fluorescent inhibitor probe FAM-LEED-FMK to label active caspase-13 enzymes in living cells or tissue samples. Analyze the fluorescent signal using fluorescence microscopy, a fluorescent plate reader, or by flow cytometry.

FLICA (Fluorescent Labeled Inhibitor of Caspases) probes are comprised of an inhibitor peptide sequence that binds to active caspase enzymes, a fluoromethyl ketone (FMK) moiety that results in an irreversible binding event with the enzyme, and a fluorescent tag (either carboxyfluorescein or sulforhodamine B) reporter. For a caspase-13 inhibitor, the enzyme recognition sequence is leucine-glutamic acid-glutamic acid-aspartic acid (LEED). The FLICA probe interacts with the enzymatic reactive center of an activated caspase via the recognition sequence, forming a covalent thioether adduct with the enzyme through the FMK moiety. FLICA probes are cell permeant and non-cytotoxic. Unbound FAM- FLICA reagent is washed away; the remaining green fluorescent signal is a direct measure of caspase-13 activity at the time the probe was added. Detection of nuclear morphology is also possible with the additional kit components Hoechst 33342 and Propidium Iodide.