Go Back Red FLICA Poly Caspases Assay Kit--SR-VAD-FMK

Product Detail

Detect active caspases with the red SR FLICA Poly-Caspases Assay Kit. This in vitro assay employs the fluorescent inhibitor probe SR-VAD-FMK to label active caspase enzymes in living cells or tissue samples. Analyze the fluorescent signal using fluorescence microscopy, a fluorescent plate reader, or by flow cytometry (green or yellow laser required).

FLICA (Fluorescent Labeled Inhibitor of Caspases) probes are comprised of an inhibitor peptide sequence that binds to active caspase enzymes, a fluoromethyl ketone (FMK) moiety that results in an irreversible binding event with the enzyme, and a fluorescent tag (either carboxyfluorescein or sulforhodamine B) reporter. For a poly-caspase inhibitor, the multi-enzyme recognition sequence is valine-alanine-aspartic acid (VAD). The FLICA probe interacts with the enzymatic reactive center of an activated caspase via the recognition sequence, forming a covalent thioether adduct with the enzyme through the FMK moiety.

FLICA probes are cell permeant and non-cytotoxic. Unbound SR- FLICA reagent is washed away; the remaining red fluorescent signal is a direct measure of caspase activity at the time the probe was added. Detection of nuclear morphology is also possible with the additional kit component, Hoechst 33342.