Go Back Green FLICA Caspase 8 Assay Kit--FAM-LETD-FMK

Product Detail

Detect active caspase-8 with the FAM FLICA Caspase 8 Assay Kit. This in vitro assay employs the fluorescent inhibitor probe FAM-LETD-FMK to label active caspase-8 enzymes in living cells or tissue samples. Analyze the fluorescent signal using fluorescence microscopy, a fluorescent plate reader, or by flow cytometry.

FLICA (Fluorescent Labeled Inhibitor of Caspases) probes are comprised of an inhibitor peptide sequence that binds to active caspase enzymes, a fluoromethyl ketone (FMK) moiety that results in an irreversible binding event with the enzyme, and a fluorescent tag (either carboxyfluorescein or sulforhodamine B) reporter. For a caspase-8 inhibitor, the enzyme recognition sequence is leucine-glutamic acid-threonine-aspartic acid (LETD). The FLICA probe interacts with the enzymatic reactive center of an activated caspase via the recognition sequence, forming a covalent thioether adduct with the enzyme through the FMK moiety.

FLICA probes are cell permeant and non-cytotoxic. Unbound FAM- FLICA reagent is washed away; the remaining green fluorescent signal is a direct measure of caspase-8 activity at the time the probe was added. Detection of nuclear morphology is also possible with the additional kit components Hoechst 33342 and Propidium Iodide.