Go Back anti-p53 Ac-K120(human)antibody, monoclonal(10E5)

Product Detail

p53 mutants are found in more than half of human cancers and are considered the most important human cancer related gene. p53 is a 53kD protein and is composed of 393 amino acids. In the unstressed normal cells, the p53 level is low and it is inactive. However, with stress, especially with DNA damage, it is activated to promote cell cycle arrest and repair of DNA damage, or induction of apoptosis. The functions and stability of p53 are regulated by phosphorylation of serine and threonine, and acetylation of lysine at various sites in the molecule. Acetylation of lysine 120 (acetyl-K120) of p53 occurs rapidly after DNA damage and is catalyzed by the MYST family acetyltransferases hMOF and TIP60, and activates transcription of proapoptotic genes, BAX and PUMA (ref 1, 2 & 3). This product is the purified IgG fraction obtained from serum-free culture medium of mouse hybridoma (clone 10E5), which produces monoclonal antibody that specifically recognizes human p53 protein with acetylated Lys120.

Applications • Western blotting (~1 ug/ml*) • ELISA • Immunoprecipitation