Go Back Cyclic GMP Direct EIA kit (One Plate)

Product Detail

Measure cGMP in lysed cells, saliva, plasma, urine or TCM

Species Independent

Measure to less than 0.05 pmol/sample

Consistent results from monoclonal primary antibody

The Cyclic GMP (cGMP) Immunoassay kit is designed to quantitatively measure cGMP present in lysed cells, plasma, urine, saliva and tissue culture media samples.

The kit is unique in that all samples and standards are diluted into an acidic Sample Diluent which contains special additives and stabilizers designed to lyse cells for measurement. AcGMP standard is provided to generate a standard curve for the assay. Aspecial Plate Primer is added to all the wells and stan�dards or samples in Sample Diluent are pipetted into the primed clear microtiter plate. AcGMP-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of an antibody to cGMP. After 2 hours, the plate is washed and substrate is added. The substrate reacts with the bound cGMP-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450nm wavelength. The concentration of cGMP in the sample is cal�culated, after making suitable correction for the dilutions. An optional Acetylation Format allows super low concentrations of cGMP to be measured.