Go Back Cyclic AMP Direct EIA Kit (One Plate)

Product Detail

Measure cAMP in lysed cells, tissue , plasma, saliva, urine or TCM

Measure to less than 4 fmol cAMP per sample

High optical density in 2.5 hours

This Cyclic AMP (cAMP) Immunoassay kit is designed to quantitatively measure cAMP present in lysed cells, tissue, plasma, urine, saliva and tissue culture media samples. The kit is unique in that all samples and standards are diluted into an acidic sample diluent which contains special additives and stabilizers designed to lyse cells for measurement. AcAMP standard is pro�vided to generate a standard curve for the assay. A special plate primer is added to all the wells and standards or samples in diluent are pipetted into the primed clear microtiter plate. AcAMP-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of an antibody to cAMP. After 2 hours, the plate is washed and substrate is added. The substrate reacts with the bound cAMP-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450nm wavelength. The concentration of cAMP in the sample is calculated, after making suitable correction for the dilutions. An optional Acetylation Format allows super low concentrations of cAMP to be measured.