Go Back Cortisone Chemiluminescent CLIA kit (One Plate)

Product Detail

The Cortisone Immunoassay kit is designed to quantitatively measure cortisone present in se-rum, urine, saliva, dried fecal extracts and tissue culture media samples. A cortisone standard is provided to generate a standard curve for the assay. Standards or diluted samples are pipetted into a white microtiter plate coated with an antibody to capture rabbit IgG. A cortisone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to cortisone to each well. After a 2 hour incubation, the plate is washed and the supplied chemiluminescent substrate is added. The substrate reacts with the bound cortisone-peroxidase conjugate and the intensity of the generated light emission is detected in a microtiter plate reader. The concentration of the cortisone in the sample is cal-culated, after making suitable correction for the dilution of the sample, using software available with most plate readers.

Serum, urine, saliva, dried fecal extracts and tissue culture media samples.