Go Back Red/ET Enhanced Flp Expression Plasmid:707-FLPe, tet-selection
Product Detail
| Cat # | A104 |
| Size | 0.2ug/ul,20ul |
| Price |
$357.50 |
The expression plasmid enables FLP-mediated site specific recombination. The plasmid confer tetracycline (707-FLPe) and propagates at 30 C in low copy number. flp expression is under control of the themosensitive cI578 promoter and takes place at 37 C - 42 C. A temperature shift up from 30 C to 37 C results in a transient FLPe recombineering activity since the expression plasmids are no longer replicated due to their pSC101-based origin and finally get lost. Compared to the wild type FLP protein FLPe has an improved thermostability and shows enhanced recombinase activity at 37 C - 40 C.