Quick & Easy Red/ET E. coli Gene Deletion Kit (need license to buy)
For fast knockouts or modifications of genes on the E.coli chromosome. Red/ET recombination allows the exchange of genetic information in a base pair precise, specific, and faithful manner. An FRT-flanked kanamycin resistance marker cassette is supplied with the kit which can be used to replace a gene on the E.coli chromosome. Red/ET recombination can replace fragments as large as 30kb from the E.coli chromosome. The use of a FRT-flanked resistance cassette for the replacement of the targeted gene allows the subsequent removal of the selection marker by a FLP-recombinase step, if required. FLP expression plasmids can be purchased. Please Note: This product is only available to licensed customers. Academic and non-profit laboratories can obtain a license free-of-charge by simply signing off on the terms of the Red-ET Non-Commercial License (.pdf file can be found on the right column of this page). Companies need to contact Gene Bridges directly regarding licensing terms (link can be found under Suppliers). Please contact B-Bridge International or Gene Bridges for further information.