Go Back Quick & Easy Red/ET Conditional Knock Out Kit - FRT (need license to buy)

Product Detail

For fast and simple integration of FRT-sites into large vector plasmids at any intended position (for generation of transgenic mouse models). The FRT-PGK-gb2-neo-FRT cassette is designed to allow neomycin / kanamycin selection in prokaryotic and eukaryotic cells. It combines a prokaryotic promoter (gb2) for expression of kanamycin resistance in E.coli with a eukaryotic promoter (PGK) for expression of neomycin resistance in mammalian cells. The included pCI-FLPe expression plasmid is coding for an improved FLP recombinase which shows a four to tenfold higher recombinational activity compared to the wildtype FLP enzyme at temperatures between 37 C (optimal growth temperature for E.coli) and 40 C (mouse body temperature). This kit can also be used to work on bacterial artificial chromosomes. High Red/ET efficiency plus convenient removal of the Red/ET Recombination protein expression plasmid pRedET after recombination.

Please Note: This product is only available to licensed customers. Academic and non-profit laboratories can obtain a license free-of-charge by simply signing off on the terms of the Red-ET Non-Commercial License (.pdf file can be found on the right column of this page). Companies need to contact Gene Bridges directly regarding licensing terms (link can be found under Suppliers). Please contact B-Bridge International or Gene Bridges for further information.